Sézary syndrome with CD3 + CD4 + CD8 − immunophenotype in a Bichon Frise

Double-positive CD4 and CD8 Sézary syndrome

CASE REPORT An 83-year-old man presented to our clinic with pruritic skin lesions of 7 months’ duration. The physical examination revealed erythroderma, nonscarring diffuse alopecia, palmoplantar keratoderma, onychodystrophy, edema of the lower limbs, and ectropion, without lymphadenopathy or weight loss (Fig 1). His medical history included hypertension and diabetes mellitus. He denied previou...

تاثیر یک جلسه درس تربیت بدنی دانشگا هها بر غلظت CD3, CD4, CD8 و CBC پلاسما

Afibrinogenemia and a circulating antibody against fibrinogen in a Bichon Frise dog.

A 1.5-year-old female Bichon Frise dog was evaluated for a life-threatening hemorrhagic condition that occurred after ovariohysterectomy, requiring 4 whole-blood transfusions. A hemostatic profile, including activated clotting time (ACT), one-stage prothrombin time (OSPT), activated partial thromboplastin time (APTT), buccal mucosal bleeding time, and specific assays (heat-precipitation microhe...

Analysis of the functional capabilities of CD3+CD4-CD8- and CD3+CD4+CD8+ human T cell clones.

The functional capabilities of human peripheral blood CD3+CD4-CD8- and CD3+CD4+CD8+ T cell clones were examined. The clones were generated by culturing purified populations of CD3+CD4-CD8- and CD3+CD4+CD8+ T cells at limiting dilution (0.3 cell/well) in the presence of PHA, rIL-2, and irradiated PBMC as feeders. Twelve CD3+CD4-CD8- and 5 CD3+CD4+CD8+ clones were generated. Clonality was documen...

Differentiation of thymocytes from CD3-CD4-CD8- through CD3-CD4-CD8+ into more mature stages induced by a thymic stromal cell clone.

We have investigated the capacity of our established thymic stromal cell clone (MRL104.8a) or its derived factor(s) to induce the differentiation of immature thymocytes. Culture of purified adult murine double-negative (CD4-CD8-, indicated here as CD4-8-) thymocytes on the MRL104.8a thymic stromal cell monolayer for 1 day resulted in the induction of an appreciable percentage of CD4-8+ thymocyt...